Journal: Frontiers in Immunology

Article Title: A fully human IgG1 antibody targeting MICA α1 domain inhibits interaction with NKG2D and activates immune effector functions against MICA-expressing cells

doi: 10.3389/fimmu.2026.1740184

Figure Lengend Snippet: Antibody-dependent cell phagocytosis mediated by anti-MICA c65 antibody. (A) Dot plots showing phagocytosis by U937-EGFP macrophages. GES-1 cells labeled with the fluorescent probe TFL4 (APC) were co-cultured with U937-EGFP macrophages (FITC) in the presence of anti-MICA c65 antibody, anti-CD20 antibody as an isotype control, or vehicle (PBS) for 2 h Assays at 4 °C served as temperature controls. Dot plots illustrate the percentage of double-positive cells, corresponding to macrophages that phagocytosed GES-1 cells. (B) Bar graph of double-positive cell percentages. Bar graph indicates the mean ± SD of the percentage of APC+ (GES-1) cells on the FITC+ (macrophages) population, compared to 37 °C PBS control, from four independent experiments. *p<0.05, analyzed by Kruskal-Wallis test. (C) Confocal microscopy images of phagocytosed GES-1 cells labeled with CellTracker Orange and co-cultured with U937-EGFP macrophages (green) for 2 h in the presence of anti-MICA c65 antibody or isotype control. Two representative fields per condition are shown. White arrows highlight phagocytosed GES-1 cells. Scale bar: 10 µm.

Article Snippet: U937-EGFP cell line, derived from human promonocytic myeloid leukemia (ATCC ® CRL-1593.2, USA), was treated with 10 ng/mL phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, USA) for 48 h to induce macrophage differentiation, following established protocols ( ).

Techniques: Labeling, Cell Culture, Control, Confocal Microscopy

Journal: bioRxiv

Article Title: The targeted cytosolic degradation of class I histone deacetylases is essential for efficient alphaherpesvirus replication

doi: 10.64898/2026.01.02.697337

Figure Lengend Snippet: (A) qRT-PCR analysis validation of E3 ligase knockdown efficiency (shPIRH2, shKCTD11, shMDM2, shCHFR, shUHRF1, shTRIM46) in HeLa cells. Data are mean ± SEM; n = 3. *** P < 0.001. (B) Immunoblotting analysis of HDAC1/2 stability in E3 ligase-knockdown HeLa cells infected with HSV-1 (MOI = 1). (C) Co-IP assay of endogenous HDAC1-MDM2 interaction in HSV-1-infected HeLa cells (MOI = 1; 24 hpi). (D) Co-IP assay of FLAG-HDAC1 with EGFP-MDM2 (WT or ΔRING) in HSV-1-infected HeLa cells (MOI = 1; 24 hpi). (E) Co-IP assay of FLAG-HDAC1 (WT or K74R) with EGFP-MDM2 in HSV-1-infected HeLa cells (MOI = 1; 24 hpi). (F) Ubiquitination assays of FLAG-HDAC1 (WT or K74R) co-expressed with EGFP-MDM2 (WT or ΔRING) in HSV-1-infected HeLa cells (MOI = 1; 24 hpi).

Article Snippet: Antibodies against CHK1 (25887-1-AP), CHK2 (13954-1-AP), RAD51 (14961-1-AP), β-actin (66009-1-lg), P53 (10442-1-AP), HDAC1 (10197-1-AP), HDAC2 (12922-3-AP), HDAC4 (17449-1-AP), HDAC6 (12834-1-AP), HDAC11 (67949-1-Ig), and EGFP (50430-2-AP) were purchased from Proteintech; antibodies against p-P53 (9286), p-ATM (13050), ATM (2873), ATR (13934), p-ATR (2853), p-CHK1 (12302), p-CHK2 (2197), γ-H2AX (80312), H3 (4499), H4K8ac (2594), H4K12ac (13944), H4 (13919), H2AX (7631), H3K9ac (4658), H3K27ac (8173), UB (20326), and MDM2 (86934) were purchased from Cell Signaling Technology; H3K56ac antibody (07-677) was purchased from Millipore; ICP4 antibody (ab6514) was purchased from Abcam; ICP0 antibody (sc-53070) was purchased from Santa Cruz Biotechnology; FLAG antibody (F7425) was purchased from Sigma-Aldrich; HA antibody (A00169) was purchased from GenScript.

Techniques: Quantitative RT-PCR, Biomarker Discovery, Knockdown, Western Blot, Infection, Co-Immunoprecipitation Assay, Ubiquitin Proteomics